Finds the best local alignments between two sequences qualifiers for parameter: asequence qualifiers for parameter: bsequence Matrix file This is the scoring matrix file used when comparing sequences. By default it is the file 'EBLOSUM62' (for proteins) or the file 'EDNAFULL' (for nucleic sequences). These files are found in the 'data' directory of the EMBOSS installation. Number of alternative matches This sets the number of alternative matches output. By default only the highest scoring alignment is shown. A value of 2 gves you other reasonable alignments. In some cases, for example multidomain proteins of cDNA and gemomic DNA comparisons, there may be other interesting and significant alignments. Gap penalty The gap penalty is the score taken away when a gap is created. The best value depends on the choice of comparison matrix. The default value of 14 assumes you are using the EBLOSUM62 matrix for protein sequences, or a value of 16 and the EDNAFULL matrix for nucleotide sequences. Gap length penalty The gap length, or gap extension, penalty is added to the standard gap penalty for each base or residue in the gap. This is how long gaps are penalized. Usually you will expect a few long gaps rather than many short gaps, so the gap extension penalty should be lower than the gap penalty. An exception is where one or both sequences are single reads with possible sequencing errors in which case you would expect many single base gaps. You can get this result by setting the gap penalty to zero (or very low) and using the gap extension penalty to control gap scoring. Start the web service and receive the result. Blocks until job is finished. Start the web service and receive a job-id. Returns immediately. Wait until a job (by job-id) has finished. Blocks until job is finished. Get status information about a running job. Returns immediately. Get the results of a job (by job-id) Start the web service and receive the result. Blocks until job is finished. Start the web service and receive a job-id. Returns immediately. Wait until a job (by job-id) has finished. Blocks until job is finished. Get status information about a running job. Returns immediately. Get the results of a job (by job-id)